{"id":955,"date":"2022-01-19T10:23:45","date_gmt":"2022-01-19T10:23:45","guid":{"rendered":"https:\/\/cytometry-bsac.be\/?p=955"},"modified":"2023-01-30T15:41:47","modified_gmt":"2023-01-30T15:41:47","slug":"molecular-biology-and-cytometry-course","status":"publish","type":"post","link":"https:\/\/cytometry-bsac.be\/index.php\/2022\/01\/19\/molecular-biology-and-cytometry-course\/","title":{"rendered":"MB&#038;C22 meeting"},"content":{"rendered":"<h2>MB&amp;C22 Meeting<\/h2>\n<p><strong>Workshops flowcytometry<\/strong><br \/>\n<strong>Basic<\/strong><br \/>\n1) Flow Cytometry &#8211; Set-up van een protocol en standaardisatie van een cytometer &#8211; Eliane Henri (Analis)<br \/>\n2) Flow cytometry of tissue biopsies &#8211; Bernard Husson (H\u00f4pital de Jolimont)<br \/>\n3) Start validation of a novel flowcytometer &#8211; Malicorne Buysse (UZG)<br \/>\n4) Blood and bone marrow differentiation by flowcytometry &#8211; Wim Renmans (UZB)<br \/>\n5) Quality assurance in flow cytometry: discussion of guidelines &#8211; Andr\u00e9 Gothot (CHU Li\u00e8ge)<br \/>\n6) Switch from 8 to 12 colors \u2013 Jan Emmerechts (AZ St-Jan)<\/p>\n<p><strong>Advanced<\/strong><br \/>\n1) Start-up with spectral cytometry \u2013 Cytotek<br \/>\n2) Flow cytometric MRD applications in hemato-oncology &#8211; Barbara Denys (UZG)<br \/>\n3) datamining and machine learning in flowcytometrie &#8211; Sofie Van Gassen (UGent)<br \/>\n4) DfN approach versus LAIP by MRD AML &#8211; Ang\u00e8le Kelder\/ Sander Snel\/Jacqueline Cloos\/ Lok Lam Ngai (CCA Amsterdam, VUmc)<br \/>\n5) Multicolour panel design: tips &amp; tricks &#8211; Gert Vanisterdael (Ugent)<br \/>\n6) Digital microscopy of bone marrow and body fluids &#8211; West medica<br \/>\n7) Case presentations \u2013 multicenter (Brigitte Cantinieaux\/Jan Emmerechts\/Ronald Malfait\/Katrien Vermeulen\/Barbara Depreter)<br \/>\nOverview Workshop Sessions<br \/>\nWorkshops Molecular Biology<\/p>\n<p><strong>Basic<\/strong><br \/>\n1) Massive Parallel Sequencing &#8211; Ben Caljon (VUB)<br \/>\n2) Statistical methods in validation reports and QC &#8211; Jeremie Gras (IPG)<br \/>\n3) qPCR assay and primer design &#8211; TBC<br \/>\n4) Bio-informatics for dummies \u2013 Paco Hulpiau &amp; Cedric Hermans<\/p>\n<p><strong>Advanced<\/strong><br \/>\n1) NGS variant interpretation + tools (SeqOne, OncoKDM, perianDx, QCI) user experience with these software &#8211; Guy Froyen (Jessa Hospital), Sara Vander Boght (UZG)<br \/>\n2) Chimerism evaluation by NGS &#8211; Helena Devos (AZ Sint-Jan)<br \/>\n3) Copy number variation by shallow sequencing in CLL and MM &#8211; Barbara Cauwelier (AZ Sint-Jan)<br \/>\n4) Interpretation of clonality analyses (Ig &amp; TCR) and IgH hypermutation analysis &#8211; Elke Boone (AZ Delta) &amp; Sabine Franke (CHU Li\u00e8ge)<br \/>\n5) RNA sequencing for the detection of fusion transcripts &#8211; Joni Van Der Meulen (UZ Gent); Claude Van Campenhout (H\u00f4pital Erasme) ; Ken Maes (UZ Brussel)<br \/>\n6) Analysis and visualization of sequencing data &#8211; Paco Hulpiau (UGent)<br \/>\n7) Covid testing &#8211; from low to high throughput &#8211; Jasmine coppens (UZA) &amp; Laurent Gillet (ULi\u00e8ge)<br \/>\n8) Bioinformatics workshop on sequencing introducing data formats, analysis and visualization &#8211; Paco Hulpiau (UGent) &amp; Marieke Mispelaere<\/p>\n<p><a href=\"https:\/\/cytometry-bsac.be\/wp-content\/uploads\/2022\/02\/Preliminary-Program-MBC-2022-18012022-1.pdf\" role=\"button\"><br \/>\nProgram<br \/>\n<\/a><\/p>\n<h2>MB&amp;C22 Meeting<\/h2>\n<p><iframe src=\"https:\/\/docs.google.com\/viewer?url=https:\/\/cytometry-bsac.be\/wp-content\/uploads\/2022\/11\/220512_A_Plenary-session_1_Evolution-in-molecular-techniques_Karl-Vandepoele.pdf&amp;embedded=true\" style=\"display: block; margin-left: auto; margin-right: auto; width: 640px; height: 325px;\" frameborder=\"1\" marginheight=\"0px\" marginwidth=\"0px\" allowfullscreen=\"\"><\/iframe><br \/>\n<iframe src=\"https:\/\/docs.google.com\/viewer?url=https:\/\/cytometry-bsac.be\/wp-content\/uploads\/2022\/11\/220512_A_Plenary-session_4_Large-scale-whole-genome-sequencing-of-metastatic-cancers_Paul-Roepman.pdf&amp;embedded=true\" style=\"display: block; margin-left: auto; margin-right: auto; width: 640px; height: 325px;\" frameborder=\"1\" marginheight=\"0px\" marginwidth=\"0px\" allowfullscreen=\"\"><\/iframe><br \/>\n<iframe src=\"https:\/\/docs.google.com\/viewer?url=https:\/\/cytometry-bsac.be\/wp-content\/uploads\/2022\/11\/220512_B_Parallel-Sessions_2_Nanopore-Sequencing_Toon-Janssen.pdf&amp;embedded=true\" style=\"display: block; margin-left: auto; margin-right: auto; width: 640px; height: 325px;\" frameborder=\"1\" marginheight=\"0px\" marginwidth=\"0px\" allowfullscreen=\"\"><\/iframe><br \/>\n<iframe 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width: 640px; height: 325px;\" frameborder=\"1\" marginheight=\"0px\" marginwidth=\"0px\" allowfullscreen=\"\"><\/iframe><br \/>\n<iframe src=\"https:\/\/docs.google.com\/viewer?url=https:\/\/cytometry-bsac.be\/wp-content\/uploads\/2022\/11\/220513_D_workshop-4.1-Gothot.pdf&amp;embedded=true\" style=\"display: block; margin-left: auto; margin-right: auto; width: 640px; height: 325px;\" frameborder=\"1\" marginheight=\"0px\" marginwidth=\"0px\" allowfullscreen=\"\"><\/iframe><br \/>\n<iframe src=\"https:\/\/docs.google.com\/viewer?url=https:\/\/cytometry-bsac.be\/wp-content\/uploads\/2022\/11\/220513_D_workshop-4.4-_Jeremie-Gras.pdf&amp;embedded=true\" style=\"display: block; margin-left: auto; margin-right: auto; width: 640px; height: 325px;\" frameborder=\"1\" marginheight=\"0px\" marginwidth=\"0px\" allowfullscreen=\"\"><\/iframe><br \/>\n<iframe 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[&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":960,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"nf_dc_page":"","_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"om_disable_all_campaigns":false,"_uag_custom_page_level_css":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"site-sidebar-layout":"no-sidebar","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center 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